AIM： To elucidate the biological effects of transforming growth factor-β1 （TGF-β1） on intrahepatic cholangiocarcinoma （ICC）.METHODS： We investigated the effects of TGF-β1 on human ICC cell lines （HuCCT1, MEC...AIM： To elucidate the biological effects of transforming growth factor-β1 （TGF-β1） on intrahepatic cholangiocarcinoma （ICC）.METHODS： We investigated the effects of TGF-β1 on human ICC cell lines （HuCCT1, MEC, and HUH-28） by monitoring the influence of TGF-β1 on tumor growth and interleukin-6 （IL-6） expression in ICC cells.RESULTS： All three human ICC cell lines produced TGF-β1 and demonstrated accelerated growth in the presence of TGF-β1 with no apoptotic effect. Studies on HuCCT1 revealed a TGF-β1-induced stimulation of the expression of TGF-β1, as well as a decrease in TGF-β1 mRNA expression induced by neutralizing anti-TGF-β1 antibody. These results indicate that TGF-β1 stimulates the production and function of TGF-β1 in an autocrine fashion. Further, IL-6 secretion was observed in all three cell lines and exhibited an inhibitory response to neutralizing anti-TGF-β1 antibody. Experiments using HuCCT1 revealed a TGF-β1-induced acceleration of IL-6 protein expression and mRNA levels. These findings demonstrate a functional interaction between TGF-β1 and IL-6. All three cell lines proliferated in the presence of IL-6. In contrast, TGF-β1 induced no growth effect in HuCCT1 in the presence of small interfering RNA against a specific cell surface receptor of IL-6 and signal transducer and activator of transcription-3.CONCLUSION： ICC cells produce TGF-β1 and confer a TGF-β1-induced growth effect in an autocrine fashion.TGF-β1 activates ILo6 production, and the functional interaction between TGF-β1 and IL-6 contributes to ICC cell growth by TGF-β1.展开更多
Mucinous carcinoma of the breast has histologically distinct features and constitutes 1-3% of all breast cancers. It produces variable amounts of extracellular epithelial mucin and clinically its prognosis is far bett...Mucinous carcinoma of the breast has histologically distinct features and constitutes 1-3% of all breast cancers. It produces variable amounts of extracellular epithelial mucin and clinically its prognosis is far better than for other types of invasive carcinoma. On the other hand, coarse calcification is a common mammographic feature of benign tumors. There have been few reported cases of breast cancer containing coarse calcification. We present a unique 40 year-old female case of mucinous carcinoma of the breast accompanied by coarse calcification on mammography, which was mimicking fibroadenoma and an unusual variant of breast cancer. This case illustrates the importance of bearing in mind that mucinous carcinoma may demonstrate coarse calcification.展开更多
Background:The efficacy of the 13C-urea breath test(UBT)for diagnosis of Helicobacter pylori(H.pylori)infection in the remnant stomach after surgery is a matter of controversy.We report a simple and accurate method of...Background:The efficacy of the 13C-urea breath test(UBT)for diagnosis of Helicobacter pylori(H.pylori)infection in the remnant stomach after surgery is a matter of controversy.We report a simple and accurate method of 13C-UBT for diagnosis of H.pylori infection in the remnant stomach after gastrectomy.Methods:Eighty patients who had undergone gastrectomy with or without subsequent H.pylori eradication therapy were examined a total of 134 times for H.pylori infection by the 13C-UBT.13C-Urea,10 mg per test,was used in powdered form or in the form of film-coated tablets.Breath samples were collected before and 10,20,and 30 min after ingestion.Mucosal biopsy specimens for bacterial culture and histological examination of the remnant stomach were collected endoscopically after each 13C-UBT test.Results:Factors that confounded the 13C-UBT results in the remnant stomach were oral bacteria,posture,and residual food.Lying horizontally on the left side was the best position,and film-coated tablets indicated no necessity for use of mouthwash.The method of anastomosis had no significant effect on the results of the 13C-UBT.Thirty minutes and a cutoff of 4.5 were optimal conditions for detection of H.pylori in the remnant stomach.Under these conditions,sensitivity,specificity,and accuracy were 79.4%(27/34 cases),95.7%(44/46 cases),and 88.8%(71/80 cases),respectively,in ordinary H.pylori diagnosis,and 100%(2/2 cases),93.3%(14/15 cases),and 94.1%(16/17 cases),respectively,in evaluating eradication at 4 weeks after treatment of H.pylori.Conclusions:Having the patient lie horizontally on the left side,using a film-coated 13C-urea tablet without using mouthwash,and measurement at 30 min provided a simple and accurate method of 13C-urea breath test for detection of H.pylori in the remnant stomach after gastrectomy.展开更多
基金Supported by the Ministry of Education, Science, Sports, and Culture of Japan, through a Grant-in-Aid for Scientific Research (C)
文摘AIM： To elucidate the biological effects of transforming growth factor-β1 （TGF-β1） on intrahepatic cholangiocarcinoma （ICC）.METHODS： We investigated the effects of TGF-β1 on human ICC cell lines （HuCCT1, MEC, and HUH-28） by monitoring the influence of TGF-β1 on tumor growth and interleukin-6 （IL-6） expression in ICC cells.RESULTS： All three human ICC cell lines produced TGF-β1 and demonstrated accelerated growth in the presence of TGF-β1 with no apoptotic effect. Studies on HuCCT1 revealed a TGF-β1-induced stimulation of the expression of TGF-β1, as well as a decrease in TGF-β1 mRNA expression induced by neutralizing anti-TGF-β1 antibody. These results indicate that TGF-β1 stimulates the production and function of TGF-β1 in an autocrine fashion. Further, IL-6 secretion was observed in all three cell lines and exhibited an inhibitory response to neutralizing anti-TGF-β1 antibody. Experiments using HuCCT1 revealed a TGF-β1-induced acceleration of IL-6 protein expression and mRNA levels. These findings demonstrate a functional interaction between TGF-β1 and IL-6. All three cell lines proliferated in the presence of IL-6. In contrast, TGF-β1 induced no growth effect in HuCCT1 in the presence of small interfering RNA against a specific cell surface receptor of IL-6 and signal transducer and activator of transcription-3.CONCLUSION： ICC cells produce TGF-β1 and confer a TGF-β1-induced growth effect in an autocrine fashion.TGF-β1 activates ILo6 production, and the functional interaction between TGF-β1 and IL-6 contributes to ICC cell growth by TGF-β1.
文摘Mucinous carcinoma of the breast has histologically distinct features and constitutes 1-3% of all breast cancers. It produces variable amounts of extracellular epithelial mucin and clinically its prognosis is far better than for other types of invasive carcinoma. On the other hand, coarse calcification is a common mammographic feature of benign tumors. There have been few reported cases of breast cancer containing coarse calcification. We present a unique 40 year-old female case of mucinous carcinoma of the breast accompanied by coarse calcification on mammography, which was mimicking fibroadenoma and an unusual variant of breast cancer. This case illustrates the importance of bearing in mind that mucinous carcinoma may demonstrate coarse calcification.
文摘Background:The efficacy of the 13C-urea breath test(UBT)for diagnosis of Helicobacter pylori(H.pylori)infection in the remnant stomach after surgery is a matter of controversy.We report a simple and accurate method of 13C-UBT for diagnosis of H.pylori infection in the remnant stomach after gastrectomy.Methods:Eighty patients who had undergone gastrectomy with or without subsequent H.pylori eradication therapy were examined a total of 134 times for H.pylori infection by the 13C-UBT.13C-Urea,10 mg per test,was used in powdered form or in the form of film-coated tablets.Breath samples were collected before and 10,20,and 30 min after ingestion.Mucosal biopsy specimens for bacterial culture and histological examination of the remnant stomach were collected endoscopically after each 13C-UBT test.Results:Factors that confounded the 13C-UBT results in the remnant stomach were oral bacteria,posture,and residual food.Lying horizontally on the left side was the best position,and film-coated tablets indicated no necessity for use of mouthwash.The method of anastomosis had no significant effect on the results of the 13C-UBT.Thirty minutes and a cutoff of 4.5 were optimal conditions for detection of H.pylori in the remnant stomach.Under these conditions,sensitivity,specificity,and accuracy were 79.4%(27/34 cases),95.7%(44/46 cases),and 88.8%(71/80 cases),respectively,in ordinary H.pylori diagnosis,and 100%(2/2 cases),93.3%(14/15 cases),and 94.1%(16/17 cases),respectively,in evaluating eradication at 4 weeks after treatment of H.pylori.Conclusions:Having the patient lie horizontally on the left side,using a film-coated 13C-urea tablet without using mouthwash,and measurement at 30 min provided a simple and accurate method of 13C-urea breath test for detection of H.pylori in the remnant stomach after gastrectomy.
