[Objectives] The research aimed to evaluate the quality of Zhenrongdan mixture by fingerprint combining QAMS. [Methods] The quality evaluation method was established and validated with echinacoside as internal referen...[Objectives] The research aimed to evaluate the quality of Zhenrongdan mixture by fingerprint combining QAMS. [Methods] The quality evaluation method was established and validated with echinacoside as internal reference to determine the contents of other components(ferulic acid, salvianolic acid B, and icariin) according to the relative correction factor. The accuracy and feasibility of QAMS were evaluated by comparison on the results between the measured value and calculation value by external standard method and QAMS. [Results] A common pattern of characteristic fingerprint of Zhenrongdan mixture by HPLC was established. Thirteen common peaks were identified, and they account for 91% of the total peak area, and four components were verified in five batches of Zhenrongdan mixture. Good similarities with correlation coefficients higher than 0.99 were found in the fingerprints. There was no significant difference between the quantitative results of the four ingredients in the five batches by external standard method and QAMS. [Conclusions] The method of fingerprint combined with QAMS could be used for the quality control of multiple components determination and fingerprint chromatography for Zhenrongdan mixture.展开更多
The research on the application of X-ray diffraction in the quantitative analysis of Chinese medicines is rare. The main reason is that the technical problems related to the internal standard and the selection of quan...The research on the application of X-ray diffraction in the quantitative analysis of Chinese medicines is rare. The main reason is that the technical problems related to the internal standard and the selection of quantitative peaks are not well solved, and the accuracy and precision of the results are not satisfactory. This study employed the concept of mass absorption coefficient based on the internal standard method, and the full spectrum fitting and quantitative methods were used to solve the above technical problems. The sample was blended. the internal standard substance of zinc oxide was fully ground, and tablets were prepared by positive pressure method. Under certain instrumental conditions, the PXRD pattern was obtained by scanning. The percentage of gypsum fibrosum in Xiaokening tablet was obtained by quantitative analysis of full spectrum fitting internal standard by TOPAS software. The method was investigated by methodology. At the same time, the method was compared by ion chromatography, and SPSS software was used to make a significant t test on the results of the two methods. After the investigation, the average standard recovery rate of CaSO4-2H2O was 99.06%(RSD = 3.02%);and the recovery rate for simulated samples was 96.7%. The method had good specificity. After statistical analysis, there was no significant difference between the new PXRD method and the traditional method of ion chromatography.展开更多
文摘[Objectives] The research aimed to evaluate the quality of Zhenrongdan mixture by fingerprint combining QAMS. [Methods] The quality evaluation method was established and validated with echinacoside as internal reference to determine the contents of other components(ferulic acid, salvianolic acid B, and icariin) according to the relative correction factor. The accuracy and feasibility of QAMS were evaluated by comparison on the results between the measured value and calculation value by external standard method and QAMS. [Results] A common pattern of characteristic fingerprint of Zhenrongdan mixture by HPLC was established. Thirteen common peaks were identified, and they account for 91% of the total peak area, and four components were verified in five batches of Zhenrongdan mixture. Good similarities with correlation coefficients higher than 0.99 were found in the fingerprints. There was no significant difference between the quantitative results of the four ingredients in the five batches by external standard method and QAMS. [Conclusions] The method of fingerprint combined with QAMS could be used for the quality control of multiple components determination and fingerprint chromatography for Zhenrongdan mixture.
文摘The research on the application of X-ray diffraction in the quantitative analysis of Chinese medicines is rare. The main reason is that the technical problems related to the internal standard and the selection of quantitative peaks are not well solved, and the accuracy and precision of the results are not satisfactory. This study employed the concept of mass absorption coefficient based on the internal standard method, and the full spectrum fitting and quantitative methods were used to solve the above technical problems. The sample was blended. the internal standard substance of zinc oxide was fully ground, and tablets were prepared by positive pressure method. Under certain instrumental conditions, the PXRD pattern was obtained by scanning. The percentage of gypsum fibrosum in Xiaokening tablet was obtained by quantitative analysis of full spectrum fitting internal standard by TOPAS software. The method was investigated by methodology. At the same time, the method was compared by ion chromatography, and SPSS software was used to make a significant t test on the results of the two methods. After the investigation, the average standard recovery rate of CaSO4-2H2O was 99.06%(RSD = 3.02%);and the recovery rate for simulated samples was 96.7%. The method had good specificity. After statistical analysis, there was no significant difference between the new PXRD method and the traditional method of ion chromatography.
